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Browsing by Author Giacomello, Alessandro

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Issue DateTitleAuthor(s)Tutors and other contributhorsSkills
2011“POTENTIAL BIOLOGICAL MEANING AND ORIGIN OF CARDIAC PROGENITOR CELLS ISOLATED AS CARDIOSPHERES"FORTE, ELVIRAGiacomello, Alessandro; Tripodi, Marco- Intra-peritoneal injection and oral gavage on mice; organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner.
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