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- Intra-peritoneal injection and oral gavage on mice; organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner.
3339164331
3D modelling
3D vision techniques
Abdominal Imaging Clinical Radiologist
Abdominal Surgery
abilità di leadership
ABITARE
AC magnetic susceptibility
accelerator physics
Accesso al credito e sviluppo
accuracy
Accuracy of analysis
Accurate
ACTING
action planning, environmental planning and management
actuary
adaptability and team working capacity
Adaptable
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