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Browsing by Skills Agarose and polyacrylamide Gel Electrophoresis, DNA gel purification,Organisms/Systems used: E. Coli, S. Cerevisiae. Mus Musculus, Cell lines.

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Issue DateTitleAuthor(s)Tutors and other contributhorsSkills
21-Jan-2014FUNCTIONAL COMPLEMENTATION OF SIR2Δ YEAST MUTATION BY THE HUMAN ORTHOLOGOUS GENE SIRT1GAGLIO, DAVIDEVittorioso, Paola; Tripodi, MarcoWestern Blot, Northern Blot, Southern Blot,ChIP, ChIP-seq, RNA-seq; PCR, semiquantitative sqRT-PCR, Colony PCR, real time qRT-PCR.; Gene and recombinant DNA cloning, recombinant protein for heterologous expression; DNA/RNA extraction, DNA/RNA precipitation, plasmid preparation Mini, Midi and Maxi Prep, skilful in DNA/RNA/Protein handling of different organisms and cell lines.; Agarose and polyacrylamide Gel Electrophoresis, DNA gel purification,Organisms/Systems used: E. Coli, S. Cerevisiae. Mus Musculus, Cell lines.
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