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Browsing by Skills Bacterial transformation • Plasmid DNA purification (Maxiprep, Midiprep)

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Issue DateTitleAuthor(s)Tutors and other contributhorsSkills
15-May-2014LA DISPLASIA FIBROSA IN MODELLI IN VITRO E IN VIVOASTROLOGO, LETIZIABIANCO, PAOLOCell culture of adherent primary, stem cells and cell lines • Transient transfection; Lentiviral and Adenoviral vector production, titration and –mediated transduction; Genomic DNA isolation from cells and tissues • RNA extraction from cells and tissues and retrotranscription • RT-PCR, Real Time PCR • Protein extraction from cells and Western Blotting • ELISA assays; Bacterial transformation • Plasmid DNA purification (Maxiprep, Midiprep); Mouse management • Mouse manipulation
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