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Please use this identifier to cite or link to this item: http://hdl.handle.net/10805/1576

Title: MEASUREMENT OF PRIMARY BIOLOGICAL AEROSOL RESPONSIBLE FOR THE ONSET OF RESPIRATORY DISEASES
Authors: POMATA, DONATELLA
Tutor: BUIARELLI, FRANCESCA
Keywords: BIOAEROSOL, FUNGAL SPORES, ERGOSTEROL, MANNITOL, ARABITOL, MURAMIC ACID, DIPICOLINIC ACID, CONVERSION FACTORS
Issue Date: 12-Jun-2012
Abstract: Inhalation of bioaerosol produces non-specific symptoms such as irritative reactions, headache, and fatigue, and human health effects such as asthma, respiratory infections, conjunctivitis, urticaria and hypersensitivity pneumonitis. The dominant biological components of air are fungal spores, bacteria and bacterial spores, ubiquitous in outdoor air, but scarcely measured due to the inadequacy of measurement methods. The use of biomarkers as tool for the determination of fungal and bacterial contribute to bioaerosol has been often suggested. In this study ergosterol, arabitol and mannitol have been associated to fungal spores, muramic acid has been associated to bacteria and dipicolinic acid has been associated to bacterial spores, as tracers. In the present work, a new analytical chemical method for the determination of this biomarkers was developed and fungal and bacterial component of aerosol was studied in suburban/rural and in urban sites. Ergosterol, arabitol, mannitol, muramic acid and dipicolinic acid content in airborne particulate matter, even at different sizes, was determined. Literature conversion factors correlating muramic acid and dipicolinic acid masses to bacteria and bacterial spores masses were applied. Literature conversion factors and new conversion factors correlating ergosterol, arabitol, and mannitol masses to fungi mass were applied and compared each other. The fungal spore concentrations obtained were different depending on the marker utilized and we deduced that in tropical regions or in very humid regions arabitol and mannitol principally come from fungal spore, especially during wet seasons. On the contrary at our latitude, that is in our meteorological condition, and dominant vegetation type arabitol and mannitol major primary emission sources are biomass burning, sea spray and plants. Therefore we conclude that, depending on the sampling area, arabitol and mannitol can overestimate fungal spore concentrations in atmosphere. Ergosterol remains the only possible biomarker and the estimated fungal concentrations in particulate matter PM10.
URI: http://hdl.handle.net/10805/1576
Research interests: Laboratory and field testing of the annular diffusion denuder technique for measurement of phase-distributed nitrophenols in atmosphere; Evaluation of pollution in urban and remote areas; Study of PM10 and PM2.5 and PM0.1 concentrations in urban air; Study of organic components of airborne particles with carcinogenic and mutagenic properties or acute toxicity; Analysis of distribution of hydrocarbons (fingerprinting and diagnostic ratios) to identify contamination sources in the groundwater, soil and atmosphere; Study of organic components of airborne particles with allergenic, infective and toxigenic properties; NIST Intercomparison Exercise Program for Organic Contaminants in airborne particulate matter (TRIAL III).
Skills short description: Expert in: - GC/MS (EI and NICI), HPLC/UV and HPLC/MS-MS; - development of analytical methods for extraction, purification and analysis of organic pollutants of anthropogenic and natural origin; - samplings of regulated and non-regulated atmospheric pollutants;
Appears in PhD:IGIENE INDUSTRIALE E AMBIENTALE

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