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- Intra-peritoneal injection and oral gavage on mice; organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner.
Agarose and polyacrylamide Gel Electrophoresis, DNA gel purification,Organisms/Systems used: E. Coli, S. Cerevisiae. Mus Musculus, Cell lines.
biology
BIOMATERIALS, BIOCOMPATIBILITY ASSAY,
CANCER STEM CELLS
DNA/RNA extraction, DNA/RNA precipitation, plasmid preparation Mini, Midi and Maxi Prep, skilful in DNA/RNA/Protein handling of different organisms and cell lines.
Gene and recombinant DNA cloning, recombinant protein for heterologous expression
HETEROTOPIC AND ORTHOTOPIC TRANSPLANTS
informatics
ISOLATION STEM CELLS AND ADULT CELLS FROM CORDON BLOOD AND TISSUE
Licentiate in Biology (5 years ≈ BSc + MSc): UAM (University Autónoma of Madrid). Specialized (≈ MSc) in Molecular Genetics and Cell Biology.
Medicinal Chemistry
MSc in Bioinformatics and Computational Biology: UCM (University Complutense of Madrid).
Organic Chemistry
PCR, semiquantitative sqRT-PCR, Colony PCR, real time qRT-PCR.
Pharmaceutical Technologies
Pharmacology
PhD in Bioinformatics, Pasteurian Sciences School
PHENOTYPIC CHARACTERIZATION: FACS, qRT-PCR, IMMUNOFLUORESCENCE, FUNCTIONAL ASSAY, IMMUNOISTOCHEMICAL
statistics
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