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|Title: ||INSULIN-LIKE-GROWTH-FACTOR-BINDING-PROTEIN-3 (IGFBP-3) CONTRASTS MELANOMA PROGRESSION IN VITRO AND IN VIVO|
|Authors: ||ABBATE, FRANCO|
|Tutor: ||Abbate, Franco|
|Issue Date: ||5-Feb-2014|
|Abstract: ||Our previous studies, revealed a dramatic degradation of the circulating IGF binding protein-3, one of the major regulators of IGF1, in the sera of patients affected by the IV stage of melanoma. For this reason, we focused on understanding the relation between IGFBP3 and melanoma cells.
In "in vitro" experiments we found that the addition of exogenous IGFBP3 to the growth medium of metastatic melanoma cells is able to reduce their migratory and invasive potential. At the molecular level, the study of the molecular pathway involved in this process, revealed a strong dephosphorilation of AKT and GSK3β. Surprisingly, the nature of this dephosphorilation is not dependent on the IGF1 sequestration but, instead, on the activation of AKT's phosphatases that inhibit its activity. Additionally our group found an important anti-tumoral activity of IGFBP3 "in vivo". In fact mice, inoculated with melanoma cells, showed a reduction of the tumor's volume after the administration of the protein, making IGFBP3 a new candidate for therapeutic approach.|
|Research interests: ||I developed a profound interest in the biology and genetics of pathological mechanisms involved in the alteration of normal cellular physiology. I intend to study how a molecular change reflects a cellular response, affecting a gene, transcriptional factor or kinase, analyzing how and which cellular substrates are consequently deregulated. For this purpose, I also intend to take advantage of large-scale analysis tools, such as proteome-profile, microarray and “in vivo” models, as already done during my PhD.|
|Skills short description: ||Cellular biology: Cell cultures: Cultures of Human and Mouse melanoma cells, analysis of migration, invasion, apoptosis, proliferation, senescence and cell cycle. Analysis with FACS sorter. Microscopy: confocal, fluorescence and light microscopy.
Molecular biology: Vector design: primers design, digestions, ligations, cloning, RNA and DNA extraction and purification, reverse transcription, PCR, real time PCR, DNA gel analysis.
Biochemistry: Western blot, Immunoprecipitation, Ribosome analysis and profiling.
Software: Excellent skills with Macintosh and Windows operating systems. Experience in programming languages and building websites. Professional use of Microsoft Office (Word, Excel and Power Point). Professional editing of Images with Photoshop and other Imaging software.|
|Personal skills keywords: ||Cell cultures, Human, Mouse,|
Molecular Biology, Genetics, Cellular Biology
PCR, Western Blot, DNA, RNA, Assay
|Appears in PhD:||BIOLOGIA UMANA E GENETICA|
Files in This Item:
|Tesi Franco Abbate.pdf||15.25 MB||Adobe PDF|
File del Curriculum Vitae:
|CurriculumVitae.pdf|| ||119.77 kB||Adobe PDF|
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