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Please use this identifier to cite or link to this item: http://hdl.handle.net/10805/2361

Title: The effects of cannabidiol and its synergism with bortezomib in multiple myeloma cell lines. A role for transient receptor potential vanilloid type-2
Authors: LIBERATI, SONIA
Tutor: Santoni, Giorgio
Keywords: Multiple Myeloma
Cannabidiol
Bortezomib
Cancer
Issue Date: 24-Feb-2014
Abstract: Multiple myeloma (MM) is a plasma cell (PC) malignancy characterised by the accumulation of a monoclonal PC population in the bone marrow (BM). Cannabidiol (CBD) is a non-psychoactive cannabinoid with antitumoural activities, and the transient receptor potential vanilloid type-2 (TRPV2) channel has been reported as a potential CBD receptor. TRPV2 activation by CBD decreases proliferation and increases susceptibility to drug-induced cell death in human cancer cells. However, no functional role has been ascribed to CBD and TRPV2 in MM. In this study, we identified the presence of heterogeneous CD138+TRPV2+ and CD138+TRPV2- PC populations in MM patients, whereas only the CD138+ TRPV2- population was present in RPMI8226 and U266 MM cell lines. Because bortezomib (BORT) is commonly used in MM treatment, we investigated the effects of CBD and BORT in CD138+TRPV2- MM cells and in MM cell lines transfected with TRPV2 (CD138+TRPV2+). These results showed that CBD by itself or in synergy with BORT strongly inhibited growth, arrested cell cycle progression and induced MM cells death by regulating the ERK, AKT and NF-κB pathways with major effects in TRPV2+ cells. These data provide a rationale for using CBD to increase the activity of proteasome inhibitors in MM.
URI: http://hdl.handle.net/10805/2361
Appears in PhD:SCIENZE IMMUNOLOGICHE

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Tesi di dottorato in Scienze Immunologiche XXVI Ciclo.pdfTesi di dottorato in Scienze Immunologiche Ciclo XXVI_ARGOMENTO: Effetto del cannabidiolo ed il suo sinergismo con il bortezomib su linee cellulari di mieloma multiplo. Il ruolo del recettore TRPV2 2.26 MBAdobe PDF


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