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Pubblicazioni Aperte DIgitali Sapienza > Fisiologia e farmacologia "Vittorio Erspamer" > TOSSICOLOGIA >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10805/934

Title: INVOLVEMENT OF OXIDATIVE STRESS IN THE PATHOGENESIS OF SCHIZOPHRENIA:FOCUS ON NOX ENZYMES
Authors: SCHIAVONE, STEFANIA
Tutor: STEARDO, LUCA
Keywords: SCHIZOPHRENIA
OXIDATIVE STRESS
Issue Date: 2011
Abstract: The imbalance between the production of reactive oxygen species (ROS) and the cellular antioxidant defence, determines a situation called “oxidative stress”. ROS react and oxidize cellular components, such as proteins or DNA, leading to cell death and severe tissue damage. The central nervous system (CNS) is particularly sensitive to oxidative stress because of high oxygen consumption, low antioxidant defense and abundance of lipids, which are prone to oxidation. For this reason a variety of CNS diseases have an oxidative stress component. In the CNS, NOX NADPH oxidases are increasingly recognized as major sources of ROS. Among the seven existing isoforms, the presence of NOX1, NOX2, NOX3 and NOX4 transcripts has been detected in total brain samples. In addition, several immunohistological and in vitro studies have investigated the expression of NOX isoforms in specific CNS regions and cells. From these studies, it appears that NOX1, NOX2 and NOX4 are present in microglia, neurons and astrocytes. Although the physiological role of constitutive NOX expression in the brain is still poorly understood, several researches suggest that they are involved in brain development and function, but most studies indicate that an excessive production of ROS by NOX enzymes participates to the progression of various neurological and psychiatric diseases. The aim of this research project was to investigate the role of NOX2 in the pathogenesis of schizophrenia.
URI: http://hdl.handle.net/10805/934
Research interests: PSYCHIATRIC DISEASES NEUROLOGICAL DISORDERS OXIDATIVE STRESS
Skills short description: In vivo preclinical trials (on rats and mice): stereotaxical surgery and intracerebral microdialysis Relevation of neurotransmitters by HPLC method either in cerebral dyalisate or in tissues Behavioural tests: novel object test, open field test, cannabinoid tetrade test, forced swimming test (FST), elevated pluz maze test, tail suspension test. Cellular cultures (in particular primary neurons culture) RT-PCR Western Blotting ELISA Immunofluorescence on tissues and on cells Immunohistochemistry on tissues and on cells
Personal skills keywords: INTRACEREBRAL MICRODIALYSIS
BEHAVIORAL TESTS
IMMUNOHISTOCHEMISTRY
CELL CULTURE
MOLECULAR BIOLOGY
Appears in PhD:TOSSICOLOGIA

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