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Pubblicazioni Aperte DIgitali Sapienza > Biotecnologie cellulari ed ematologia > SCIENZE EMATOLOGICHE >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10805/979

Title: Evaluation of gene expression profiling in Chronic Lymphocytic Leukemia
Authors: Tavolaro, Simona
Tutor: Chiaretti, Sabina
Keywords: Gene expression profiling
Chronic Lymphocytic Leukemia
Protein Kinases
Antigenic stimulation
Issue Date: 12-Jul-2010
URI: http://hdl.handle.net/10805/979
Research interests: - Evaluation of gene expression profile of protein kinases in chronic lymphocytic leukemia in the identification of a role of second generation protein kinase inhibitors - Evaluation of TP53 mutations with p53 research array test in chronic lymphocytic leukemia: correlation with clinical outcome and gene expression profiling - MiR profiling evaluation in chronic lymphocytic leukemia and adult acute lymphoblastic leukemia - Microarray analysis in melanoma cell lines - Microarray analysis in chronic lymphocytic leukemia cells following antigenic stimulation of BCR - MiR profiling of chronic lymphocytic leukemia cells following antigenic stimulation of BCR
Skills short description: •DNA, RNA and protein extraction. •PCR, RT-PCR, Real-time PCR. •cDNA and oligo Microarray. •miR profiling. •Western Blot and Immunoprecipitation. •Cellular cultures. •Separation of cell subsets by immunomagnetic techniques. •Transfection by lipofectamine and/or Ca-phosphate methods in HepG2 and Saos cell lines. •Luciferase assay.
Personal skills keywords: Gene expression profiling
Chronic Lymphocytic Leukemia
B-cell antigenic stimulation
miR profiling
Identification of novel potential therapeutic targets
Appears in PhD:SCIENZE EMATOLOGICHE

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