Pubblicazioni Aperte DIgitali Sapienza > Biotecnologie cellulari ed ematologia > SCIENZE EMATOLOGICHE >

Please use this identifier to cite or link to this item:

Title: Evaluation of gene expression profiling in Chronic Lymphocytic Leukemia
Authors: Tavolaro, Simona
Tutor: Chiaretti, Sabina
Keywords: Gene expression profiling
Chronic Lymphocytic Leukemia
Protein Kinases
Antigenic stimulation
Issue Date: 12-Jul-2010
Research interests: - Evaluation of gene expression profile of protein kinases in chronic lymphocytic leukemia in the identification of a role of second generation protein kinase inhibitors - Evaluation of TP53 mutations with p53 research array test in chronic lymphocytic leukemia: correlation with clinical outcome and gene expression profiling - MiR profiling evaluation in chronic lymphocytic leukemia and adult acute lymphoblastic leukemia - Microarray analysis in melanoma cell lines - Microarray analysis in chronic lymphocytic leukemia cells following antigenic stimulation of BCR - MiR profiling of chronic lymphocytic leukemia cells following antigenic stimulation of BCR
Skills short description: •DNA, RNA and protein extraction. •PCR, RT-PCR, Real-time PCR. •cDNA and oligo Microarray. •miR profiling. •Western Blot and Immunoprecipitation. •Cellular cultures. •Separation of cell subsets by immunomagnetic techniques. •Transfection by lipofectamine and/or Ca-phosphate methods in HepG2 and Saos cell lines. •Luciferase assay.
Personal skills keywords: Gene expression profiling
Chronic Lymphocytic Leukemia
B-cell antigenic stimulation
miR profiling
Identification of novel potential therapeutic targets

Files in This Item:

File Description SizeFormat
Tesi dottorato Simona definitiva PDF.pdfTesi dottorato di ricerca in Scienze Ematologiche1.8 MBAdobe PDF

File del Curriculum Vitae:

CurriculumVitae.doc 64 kBMicrosoft Word

This item is protected by original copyright

Recommend this item

Items in PADIS are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback Sviluppo e manutenzione a cura del CINECA